CAMKK2 (CAMKKβ) is a member of the CAMKK family. It is broadly distributed in tissues with highest levels in brain, thymus, spleen, and testis (1). CaMKK2 undergoes intramolecular autophosphorylation, is regulated by Ca2+/calmodulin and phosphorylates CaMKI and CaMKIV on Thr177 and Thr200, respectively. CaMKK2 activates both CaMKI and CaMKIV when coexpressed in Jurkat T cells. CaMKK2 also phosphorylates and regulates the activity of AMPK, which is an important regulator of cellular metabolism in response to metabolic stress (2).
CAMKK; CAMKKB; KIAA0787; MGC15254
1. Anderson, K.A. et al: Components of a calmodulin-dependent protein kinase cascade. Molecular cloning, functional characterization and cellular localization of Ca2+/calmodulin-dependent protein kinase kinase beta.
2. Hurley, R.L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6.
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Buard Isabelle et al., CaMKII Autonomy Is Required for Initiating But Not for Maintaining Neuronal Long-Term Information Storage Journal Of Neuroscience July 2005 10.1523/JNEUROSCI.1469-10.2010
Wang Ping et al., Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2 BMC Biochemistry September 2010 10.1186/1471-2091-11-36
Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, Ser/Thr Kinases