Recombinant full-length human PDHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Catalog No. P57-10G
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
P57-10G-05 | 5 ug | $226 | |
P57-10G-10 | 10 ug | $325 | |
P57-10G-BULK | BULK | Contact Us |
Overview:
PDHK1 or Pyruvate Dehydrogenase Kinase 1 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH) (1). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK1 is an important regulator of PDH in clonal β-cells and the activity of PDHK1 and PDH are important for efficient metabolic coupling. Maintaining low PDHK1 expression/activity and keeping PDH in a dephosphorylated and active state, is important for β -cells to achieve the metabolic flux rates necessary for maximal glucose-stimulated insulin secretion (2).
Gene Aliases:
PDK1
Genbank Number:
References:
1. Gudi, R. et al: Diversity of the pyruvate dehydrogenase kinase gene family in humans. J. Biol. Chem. 270: 28989-28994, 1995.
2. Krus, U. et al: Pyruvate dehydrogenase kinase 1 controls mitochondrial metabolism and insulin secretion in INS-1 832/13 clonal beta-cells. Biochem J. 2010 Jul 1;429(1):205-13.
Specific Activity:
Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~70 kDa
Tsuchiya A et al., PI3 kinase directly phosphorylates Akt1/2 at Ser473/474 in the insulin signal transduction pathway Journal of Endocrinology January 2014 10.1530/JOE-13-0172
T Kanno et al., Combination of PKCε Activation and PTP1B Inhibition Effectively Suppresses Aβ-Induced GSK-3β Activation and Tau Phosphorylation. Molecular Neurobiology September 2016 10.1007/s12035-015-9405-x
Cancer, Cardiovascular Disease, Metabolic Disorder, Ser/Thr Kinases
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