Overview:
The PAD1 Inhibitor Screening Kit is a direct Enzyme-Linked ImmunoSorbent Assay (ELISA) kit for use in PAD1-targeted inhibitor profiling assays. The kit includes recombinant full-length human PAD1 expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag (accession number NM_013358).
Components:
Part Number | Part | Size | Storage | |
Kit Box | AB99-61DM | Detection Antibody, 500X | 25 ul/1 | Store kit box and contents at 4oC. |
CP01-506 | NeutrAvidin Coated Plate | 1 plate/1 | ||
CS01-506 | TMB Substrate | 6 ml/1 | ||
P312-09 | PAD Buffer | 8 ml/1 | ||
SS01-09 | Stop Solution | 3 ml/1 | ||
T01-09 | Trypsin Digestion Buffer | 15 ml/1 | ||
WB20-09 | Wash Buffer, 20X | 15 ml/2 | ||
Ice Pack | P312-37C | PAD Cocktail, Active | 5 ug/1 | Store product at –20oC or –70oC as specified on individual component labels. For PAD enzymes, avoid repeated handling and multiple freeze/thaw cycles. |
P312-58 | PAD Substrate | 500 ug/1 | ||
T575-31N | Trypsin, Active | 200 ug/1 | ||
D86-09 | DTT Solution | 10 ul/1 | ||
P312-310G | PAD1, Active | 50 ug/1 |
References:
1. Rothnagel, J.A., & Rogers, G.E. (1984). Citrulline in proteins from the enzymatic deimination of arginine residues. Methods Enzymol. 107, 624-31.
2. Raijmakers, R. et al. (2007). Methylation of Arginine Residues Interferes with Citrullination by Peptidylarginine Deiminases in vitro. J. Mol. Biol. 367, 1118–29.
3. Tilvawala, R., & Thompson, P.R. (2019). Peptidyl arginine deiminases: detection and functional analysis of protein citrullination. Curr. Opin. Struct. Biol. 20, 1-11.
4. Wildeman E., & Pires M.M. (2013). Facile fluorescence-based detection of PAD4-mediated citrullination. Chembiochem. 14, 963-7.
5. Clancy K.W. et al. (2016). Detection and identification of protein citrullination in complex biological systems. Curr. Opin. Chem. Biol. 30, 1-6.
Representative Data:
Representative Data Demonstration of high sensitivity and specificity of the assay achieved by using human recombinant PAD enzymes with PAD Activity Kit.
Related Products:
Name | Catalog Number | Species | Tag | Expression System | Sequence | Genbank Number |
---|---|---|---|---|---|---|
PAD Detection Kit | P312-863 | |||||
PAD Cocktail Inhibitor Screening Kit | P37C-863 | |||||
PAD2 (human) Inhibitor Screening Kit | P318BG-863 | |||||
PAD1, Active | P312-310G | Human | GST | Sf9 insect cells using baculovirus | Full length | NM_013358 |
PAD2, Active | P312-310BG | Mouse | GST | Sf9 insect cells using baculovirus | Full length | BC049947 |
PAD2, Active | P312-318BG | Human | GST | Sf9 insect cells using baculovirus | Full length | NM_007365 |
PAD3, Active | P312-310CG | Human | GST | Sf9 insect cells using baculovirus | Full length | NM_016233 |
PAD4, Active | P312-310DG | Human | GST | Sf9 insect cells using baculovirus | Full length | BC025718 |
PAD Cocktail, Active | P312-37C | GST | Sf9 insect cells using baculovirus | Full Length | NM_013358 (PAD1) | |
PAD1 Inhibitor Screening Kit | P310G-863 | |||||
PAD2 (mouse) Inhibitor Screening Kit | P310BG-863 | |||||
PAD3 Inhibitor Screening Kit | P310CG-863 | |||||
PAD4 Inhibitor Screening Kit | P310DG-863 |
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