Specificity:

Recognizes the MBP protein

Cross Reactivity:

Human, Mouse, Rat and Bovine

Host:

Chicken, IgY

Immunogen:

Three peptide sequences conserved in higher vertebrate MBP proteins

Purification:

Total IgY fraction

Stability:

Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data:

Western blot of rat cortex homogenate showing specific immunolabeling of the ~ 18 & 22kDa MBP protein.

Sample Data:

Rat mixed neuron/glial cultures stained with chicken anti-Myelin Basic Protein (green). Blue is a DNA stain. Note that the Myelin Basic Protein antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.