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Recombinant full-length untagged Bacteriophage lambda Lambda Protein Phosphatase was expressed in E. coli.
Catalog No. L01-20U
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
L01-20U-20 | 20000 U | $165 | |
L01-20U-100 | 100000 U | $615 | |
L01-20U-BULK | BULK | Contact Us |
Overview:
Lambda Protein Phosphatase (lambda PP) is a Mn 2-dependent protein phosphatase composed of 221 amino acids and is encoded on ORF211 of bacteriophage lambda (1). Lambda PP has activity towards phosphorylated serine, threonine, tyrosine and histidine residues (2). It can be inhibited by vanadate ions (3).
Genbank Number:
References:
1. Cohen, P.T.W., and Cohen, P. 1989. Discovery of a protein phosphatase activity encoded in the genome of bacteriophage lambda. Probable identity with open reading frame 221. Biochem. J. 260, 931-934.
2. Zhuo S et al. 1993. Expression, purification, crystallization, and biochemical characterization of recombinant protein phosphatase. J. Biol. Chem. 268:17754-17761.
3. Gordon JA. 1991. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 201:477-82.
Alternative name (s):
Lambda PP, Serine/Threonine-protein phosphatase
Storage and Stability:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Specific Activity:
The specific activity was determined to be 613,000 Units/ml as per the activity assay protocol.
Unit Definition:
One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50mM) in 1 minute at 30°C in a total reaction volume of 50µl.
Molecular Weight:
Calculated MW 25.2 kDa
There are no related publications available for this product.
Ser/Thr Kinases
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